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human parainfluenza virus type 1 (strain washington/20993/1964, genbank accession af016280  (ViraTree LLC)
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ViraTree LLC human parainfluenza virus type 1 (strain washington/20993/1964, genbank accession af016280
(A) Virus binding curves were generated similarly as described in the legend to (1.0 x 10 9 <t>hPIV1</t> or SeV virions). (B) The sialidase activity of different concentrations of hPIV1 and SeV (starting concentration 1.0 x 10 10 virus particles/ml) was assessed in triplicate using 4-MUNANA under standard conditions. Means and standard deviations are graphed. (C) The sialidase activity of different amounts of hPIV1 HN and SeV HN proteins were determined as described in the legend. (D) Enzyme kinetic analysis for hPIV1/SeV HN proteins was performed similarly as described in the legend. Enzyme kinetics parameters obtained from progress curve analysis for the 4-MUNANA substrate are shown in the table in the middle. (E) SA sensors loaded with saturate 3’S(LN) 3 were reacted with 1 μg hPIV1/SeV-HN proteins for 10 mins to generate the binding curve. The binding of soluble hPIV1/SeV HN proteins was undetectable in BLI analysis. (F) Streptavidin sensors were loaded to saturation with 3’S(LN) 3 receptors. Subsequently, the sensors were incubated with standard amounts of hPIV1/SeV HN-NPs. HN-NPs binding curves were generated similarly as described in the legend using 3’S(LN) 3 in the absence or presence of 1 mM BCX2798. (G) The initial binding rate of HN-NPs was determined from graphs as shown in F (n = 3, data are mean ± SD) and analyzed using one-way ANOVA test. (H) Binding of MAL I and ECA after incubation of the sensors with the indicated HN-NPs. Significance was analyzed using two-way ANOVA test. MAL I binding in the absence of sialidase activity and ECA binding after treatment of the sensors with AUNA are shown as dashed lines. **** P < 0.0001. Created with Biorender.com .
Human Parainfluenza Virus Type 1 (Strain Washington/20993/1964, Genbank Accession Af016280, supplied by ViraTree LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human parainfluenza virus type 1 strain washington/20993/1964  (ViraTree LLC)
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(A) Virus binding curves were generated similarly as described in the legend to (1.0 x 10 9 <t>hPIV1</t> or SeV virions). (B) The sialidase activity of different concentrations of hPIV1 and SeV (starting concentration 1.0 x 10 10 virus particles/ml) was assessed in triplicate using 4-MUNANA under standard conditions. Means and standard deviations are graphed. (C) The sialidase activity of different amounts of hPIV1 HN and SeV HN proteins were determined as described in the legend. (D) Enzyme kinetic analysis for hPIV1/SeV HN proteins was performed similarly as described in the legend. Enzyme kinetics parameters obtained from progress curve analysis for the 4-MUNANA substrate are shown in the table in the middle. (E) SA sensors loaded with saturate 3’S(LN) 3 were reacted with 1 μg hPIV1/SeV-HN proteins for 10 mins to generate the binding curve. The binding of soluble hPIV1/SeV HN proteins was undetectable in BLI analysis. (F) Streptavidin sensors were loaded to saturation with 3’S(LN) 3 receptors. Subsequently, the sensors were incubated with standard amounts of hPIV1/SeV HN-NPs. HN-NPs binding curves were generated similarly as described in the legend using 3’S(LN) 3 in the absence or presence of 1 mM BCX2798. (G) The initial binding rate of HN-NPs was determined from graphs as shown in F (n = 3, data are mean ± SD) and analyzed using one-way ANOVA test. (H) Binding of MAL I and ECA after incubation of the sensors with the indicated HN-NPs. Significance was analyzed using two-way ANOVA test. MAL I binding in the absence of sialidase activity and ECA binding after treatment of the sensors with AUNA are shown as dashed lines. **** P < 0.0001. Created with Biorender.com .
Human Parainfluenza Virus Type 1 Strain Washington/20993/1964, supplied by ViraTree LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant washington/20993/1964-derived strain encoding gfp  (ViraTree LLC)
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ViraTree LLC recombinant washington/20993/1964-derived strain encoding gfp
(A) Virus binding curves were generated similarly as described in the legend to (1.0 x 10 9 <t>hPIV1</t> or SeV virions). (B) The sialidase activity of different concentrations of hPIV1 and SeV (starting concentration 1.0 x 10 10 virus particles/ml) was assessed in triplicate using 4-MUNANA under standard conditions. Means and standard deviations are graphed. (C) The sialidase activity of different amounts of hPIV1 HN and SeV HN proteins were determined as described in the legend. (D) Enzyme kinetic analysis for hPIV1/SeV HN proteins was performed similarly as described in the legend. Enzyme kinetics parameters obtained from progress curve analysis for the 4-MUNANA substrate are shown in the table in the middle. (E) SA sensors loaded with saturate 3’S(LN) 3 were reacted with 1 μg hPIV1/SeV-HN proteins for 10 mins to generate the binding curve. The binding of soluble hPIV1/SeV HN proteins was undetectable in BLI analysis. (F) Streptavidin sensors were loaded to saturation with 3’S(LN) 3 receptors. Subsequently, the sensors were incubated with standard amounts of hPIV1/SeV HN-NPs. HN-NPs binding curves were generated similarly as described in the legend using 3’S(LN) 3 in the absence or presence of 1 mM BCX2798. (G) The initial binding rate of HN-NPs was determined from graphs as shown in F (n = 3, data are mean ± SD) and analyzed using one-way ANOVA test. (H) Binding of MAL I and ECA after incubation of the sensors with the indicated HN-NPs. Significance was analyzed using two-way ANOVA test. MAL I binding in the absence of sialidase activity and ECA binding after treatment of the sensors with AUNA are shown as dashed lines. **** P < 0.0001. Created with Biorender.com .
Recombinant Washington/20993/1964 Derived Strain Encoding Gfp, supplied by ViraTree LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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md 20993  (National Institute of Standards and Technology)
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National Institute of Standards and Technology md 20993
(A) Virus binding curves were generated similarly as described in the legend to (1.0 x 10 9 <t>hPIV1</t> or SeV virions). (B) The sialidase activity of different concentrations of hPIV1 and SeV (starting concentration 1.0 x 10 10 virus particles/ml) was assessed in triplicate using 4-MUNANA under standard conditions. Means and standard deviations are graphed. (C) The sialidase activity of different amounts of hPIV1 HN and SeV HN proteins were determined as described in the legend. (D) Enzyme kinetic analysis for hPIV1/SeV HN proteins was performed similarly as described in the legend. Enzyme kinetics parameters obtained from progress curve analysis for the 4-MUNANA substrate are shown in the table in the middle. (E) SA sensors loaded with saturate 3’S(LN) 3 were reacted with 1 μg hPIV1/SeV-HN proteins for 10 mins to generate the binding curve. The binding of soluble hPIV1/SeV HN proteins was undetectable in BLI analysis. (F) Streptavidin sensors were loaded to saturation with 3’S(LN) 3 receptors. Subsequently, the sensors were incubated with standard amounts of hPIV1/SeV HN-NPs. HN-NPs binding curves were generated similarly as described in the legend using 3’S(LN) 3 in the absence or presence of 1 mM BCX2798. (G) The initial binding rate of HN-NPs was determined from graphs as shown in F (n = 3, data are mean ± SD) and analyzed using one-way ANOVA test. (H) Binding of MAL I and ECA after incubation of the sensors with the indicated HN-NPs. Significance was analyzed using two-way ANOVA test. MAL I binding in the absence of sialidase activity and ECA binding after treatment of the sensors with AUNA are shown as dashed lines. **** P < 0.0001. Created with Biorender.com .
Md 20993, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Virus binding curves were generated similarly as described in the legend to (1.0 x 10 9 hPIV1 or SeV virions). (B) The sialidase activity of different concentrations of hPIV1 and SeV (starting concentration 1.0 x 10 10 virus particles/ml) was assessed in triplicate using 4-MUNANA under standard conditions. Means and standard deviations are graphed. (C) The sialidase activity of different amounts of hPIV1 HN and SeV HN proteins were determined as described in the legend. (D) Enzyme kinetic analysis for hPIV1/SeV HN proteins was performed similarly as described in the legend. Enzyme kinetics parameters obtained from progress curve analysis for the 4-MUNANA substrate are shown in the table in the middle. (E) SA sensors loaded with saturate 3’S(LN) 3 were reacted with 1 μg hPIV1/SeV-HN proteins for 10 mins to generate the binding curve. The binding of soluble hPIV1/SeV HN proteins was undetectable in BLI analysis. (F) Streptavidin sensors were loaded to saturation with 3’S(LN) 3 receptors. Subsequently, the sensors were incubated with standard amounts of hPIV1/SeV HN-NPs. HN-NPs binding curves were generated similarly as described in the legend using 3’S(LN) 3 in the absence or presence of 1 mM BCX2798. (G) The initial binding rate of HN-NPs was determined from graphs as shown in F (n = 3, data are mean ± SD) and analyzed using one-way ANOVA test. (H) Binding of MAL I and ECA after incubation of the sensors with the indicated HN-NPs. Significance was analyzed using two-way ANOVA test. MAL I binding in the absence of sialidase activity and ECA binding after treatment of the sensors with AUNA are shown as dashed lines. **** P < 0.0001. Created with Biorender.com .

Journal: PLOS Pathogens

Article Title: Unraveling dynamics of paramyxovirus-receptor interactions using nanoparticles displaying hemagglutinin-neuraminidase

doi: 10.1371/journal.ppat.1012371

Figure Lengend Snippet: (A) Virus binding curves were generated similarly as described in the legend to (1.0 x 10 9 hPIV1 or SeV virions). (B) The sialidase activity of different concentrations of hPIV1 and SeV (starting concentration 1.0 x 10 10 virus particles/ml) was assessed in triplicate using 4-MUNANA under standard conditions. Means and standard deviations are graphed. (C) The sialidase activity of different amounts of hPIV1 HN and SeV HN proteins were determined as described in the legend. (D) Enzyme kinetic analysis for hPIV1/SeV HN proteins was performed similarly as described in the legend. Enzyme kinetics parameters obtained from progress curve analysis for the 4-MUNANA substrate are shown in the table in the middle. (E) SA sensors loaded with saturate 3’S(LN) 3 were reacted with 1 μg hPIV1/SeV-HN proteins for 10 mins to generate the binding curve. The binding of soluble hPIV1/SeV HN proteins was undetectable in BLI analysis. (F) Streptavidin sensors were loaded to saturation with 3’S(LN) 3 receptors. Subsequently, the sensors were incubated with standard amounts of hPIV1/SeV HN-NPs. HN-NPs binding curves were generated similarly as described in the legend using 3’S(LN) 3 in the absence or presence of 1 mM BCX2798. (G) The initial binding rate of HN-NPs was determined from graphs as shown in F (n = 3, data are mean ± SD) and analyzed using one-way ANOVA test. (H) Binding of MAL I and ECA after incubation of the sensors with the indicated HN-NPs. Significance was analyzed using two-way ANOVA test. MAL I binding in the absence of sialidase activity and ECA binding after treatment of the sensors with AUNA are shown as dashed lines. **** P < 0.0001. Created with Biorender.com .

Article Snippet: Human parainfluenza virus type 1 (strain Washington/20993/1964, GenBank accession no. AF016280) was purchased from ViraTree. hPIV1, hPIV3 and SeV were propagated in LLC-MK2 cells using Opti-MEM (Thermo Fisher Scientific).

Techniques: Virus, Binding Assay, Generated, Activity Assay, Concentration Assay, Incubation